Excellent. That one belongs in any Python style guide. Though technically it's not a style, it does lead to better readability, and reduces the propensity for unforseen consequences.
The reason it's not in the Python style guide is because it's a symptom of other problems in code. Lists are for holding multiple values of the same type. If you know that a list will always have one and only one value, it's not conceptually a list, it's some other type that's been encoded into a list for some reason, and you should fix that conceptual mismatch rather than papering over the issue with a style idiom.
That sounds logical enough, except for two things:
First: You are often working with someone-else's library which for various reasons you cannot change.
Second: It is not uncommon to use a standard method that may well be able to return multiple items, but in your use case it should only return one. Case in point: a database call that returns the result of a query.
What you say sounds right, but it's not. Using some DB APIs you'll get back a list of results, and you'll know from the SQL that it will only have one result.
Another example is if you know there is a single item in a data structure, and use list comprehension to extract it. You'll end up with a list of one item.
But in that case, either your code is evidently in a branch where the list will have one item -- as in your example -- in which case the context makes it clear. And if there's no context, such as in a nested function call, then at some point you should be passing that lone list item into a function which takes the item as an argument.